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brightvision anti rabbit ap  (Vector Laboratories)


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    Structured Review

    Vector Laboratories brightvision anti rabbit ap
    Brightvision Anti Rabbit Ap, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+blue+alkaline+phosphatase+(blue+ap)+substrate+kit/Vector+Blue+Alkaline+Phosphatase+(Blue+AP)+Substrate+Kit/10__22203_slash_ecm__v033a11-129-57-73
    Average 96 stars, based on 1579 article reviews
    brightvision anti rabbit ap - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Activity Assay:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector® Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    In Situ:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector® Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    Negative Control:

    Article Title: A proprietary lipidosterolic extract of Serenoa repens promotes hair growth through mechanisms that extend beyond 5-alpha reductase inhibition: Insights from human hair follicle organ culture.
    Article Snippet: .. Alkaline phosphatase activity in situ was detected using the Vector® Blue Substrate Kit and alkaline phosphatase (SK5300, Biozol) according to the manufacturer's instructions, with levamisole as a negative control [39, 53]. ..

    Staining:

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia
    Article Snippet: .. After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in . ..

    Article Title: Spatial localization of avian and human influenza A virus receptors in male and female bovine reproductive tissues.
    Article Snippet: After washing the sections, slides were incubated with streptavidin-alkaline phosphatase (SA-5100-1, Vector Laboratories), diluted to 1:100 in lectin TBS for 10 minutes. .. The staining was developed by adding Vector® Blue substrate (SK-5300, Vector Laboratories) according to the manufacturer’s instructions, for 20 minutes. ..

    Article Title: Methods for treatment of cancers harboring an H3K27M mutation
    Article Snippet: .. After quenching the DAB substrate development in TBS and staining with the 14g2a primary antibody, GD2 signal was developed using a polymer-based alkaline phosphatase secondary (ImmPRESS AP anti-mouse IgG, Vector Laboratories, 30 minutes at RT) and blue alkaline phosphatase substrate (Vector Blue AP substrate kit, Vector Laboratories, 150 seconds at RT). .. AP development was quenched in TBS, and samples were mounted and imaged (Zeiss AxioObserver).

    Incubation:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: The sections were subjected to heatinduced epitope retrieval (HIER) (van der Loos, 2010), performed in a PreTreatment Module (Lab Vision) by incubation in citrate buffer pH 6.0 (TA-250-PM1X, Thermo Fisher Scientific) for 20 min at 98 °C, rinsed with tap water and incubated with Superblock (AAA999, Klinipath) for 10 min at RT. .. Sections were incubated overnight at 4 °C with rat anti-mouse monoclonal F4/80 (macrophages; MCA497GA, clone: CI:A3-1, Serotec) diluted 1: 1000 in Tris-HCl-buffered saline (TBS; 50 mM Tris, 0.9 % NaCl), subsequently with rabbit anti-rat IgG (6130-01, fab2; SBA/ITK) diluted 1: 3000 in TBS with 20 % normal mouse serum for 30 min at RT, followed by undiluted BrightVision anti-rabbit AP (DPVB-55AP, Immunologic) for 30 min at RT and finally with Vector Blue (SK-5300, Vector Labs) for 10 min at RT, with three TBS washes between all incubations. ..

    Saline:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: The sections were subjected to heatinduced epitope retrieval (HIER) (van der Loos, 2010), performed in a PreTreatment Module (Lab Vision) by incubation in citrate buffer pH 6.0 (TA-250-PM1X, Thermo Fisher Scientific) for 20 min at 98 °C, rinsed with tap water and incubated with Superblock (AAA999, Klinipath) for 10 min at RT. .. Sections were incubated overnight at 4 °C with rat anti-mouse monoclonal F4/80 (macrophages; MCA497GA, clone: CI:A3-1, Serotec) diluted 1: 1000 in Tris-HCl-buffered saline (TBS; 50 mM Tris, 0.9 % NaCl), subsequently with rabbit anti-rat IgG (6130-01, fab2; SBA/ITK) diluted 1: 3000 in TBS with 20 % normal mouse serum for 30 min at RT, followed by undiluted BrightVision anti-rabbit AP (DPVB-55AP, Immunologic) for 30 min at RT and finally with Vector Blue (SK-5300, Vector Labs) for 10 min at RT, with three TBS washes between all incubations. ..

    Polymer:

    Article Title: Methods for treatment of cancers harboring an H3K27M mutation
    Article Snippet: .. After quenching the DAB substrate development in TBS and staining with the 14g2a primary antibody, GD2 signal was developed using a polymer-based alkaline phosphatase secondary (ImmPRESS AP anti-mouse IgG, Vector Laboratories, 30 minutes at RT) and blue alkaline phosphatase substrate (Vector Blue AP substrate kit, Vector Laboratories, 150 seconds at RT). .. AP development was quenched in TBS, and samples were mounted and imaged (Zeiss AxioObserver).



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    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
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    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
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    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline <t>phosphatase-stained</t> colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.
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    Image Search Results


    Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Effect of RhiPSC culture conditions on cloning efficiency. (A) Representative images of alkaline phosphatase-stained colonies from RhiPSC line M6 clone 16.02 on mouse embryonic fibroblasts (MEFs), Matrigel, or Vitronectin in UPPS medium with either no supplement, Y-27632, or CEPT. Scale bar, 100 µm. (B) Graph depicting percent (%) cloning efficiency (y-axis) of alkaline phosphatase-positive colonies cultured on different matrices (x-axis) in UPPS medium with no supplement (circles), Y-27632 (squares), or CEPT (triangles). Individual data points correspond to the mean (dashed lines) % cloning efficiency of RhiPSC colonies counted among six technical replicates per cell line (M4 clone 14.09, red; M6 clone 16.02, pink; W4 clone 14.06, black; W6 clone 5.07, grey). Black brackets indicate statistically significant differences between two supplement types within a substrate group; black lines indicate statistically significant differences between two substrates with the same supplement. p < 0.05 is considered statistically significant. Tukey’s multiple comparisons post-hoc p-values: a=0.0488, b=0.0098, c=0.0097, d=0.0133, e=0.0177, f=0.0483, g=0.0166.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Cloning, Staining, Cell Culture

    Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Amplification, Sequencing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus

    Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Journal: bioRxiv

    Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

    doi: 10.64898/2026.03.17.712482

    Figure Lengend Snippet: Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

    Article Snippet: After 5 days, colonies were stained with a Vector Blue Alkaline Phosphatase Substrate kit (Vector Laboratories, cat. no. SK-5300) as described in .

    Techniques: Amplification, Sequencing, Mutagenesis, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus